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Fig. 1 | Virology Journal

Fig. 1

From: Serine/threonine kinases 31(STK31) may be a novel cellular target gene for the HPV16 oncogene E7 with potential as a DNA hypomethylation biomarker in cervical cancer

Fig. 1

HPV16 E7 and E6/E7 induced or influenced STK31 expression through an epigenetic mechanism and STK31 expression varied with the development of cervical tumors. a The transfection efficiency of HPV16 E6, E7及E6/E7 was tested by Western blot. SiHa: positive control. b RT-PCR and western blotting results regarding STK31 expression in the HPV16/18-positive cervical cell lines HeLa, SiHa, and CaSki and the HPV-negative CC cell lines C33A and HT-3; results of RT-PCR and western blotting results indicating that STK31 was re-expressed in the HPV16 E6 or/and E7 oncogene-transfected cells except C33AE6 and HT-3E6. c qPCR results showing that the STK31 expression levels in the C33AE6/E7 and HT-3E6/E7 cells were greater than those in the C33AE7 and HT-3E7 cells, respectively. d The MSP results were consistent with the BGS results and indicated that the STK31 promoter/exon 1 was hypermethylated in the C33A and HT-3 cell lines and hypomethylated in the HeLa, SiHa, and CaSki cell lines. Decreased methylation statuses were detected in the C33AE7, C33AE6/E7, HT-3E7, and HT-3E6/E7 cell lines, and no methylation changes were observed in the C33AE6 and HT-3E6 cells compared with the C33A-V and HT-3 V cells, respectively. e The expression of STK31 was higher in the HPV16/18-positive cases than in the HPV-negative cases of CC and CIN3 (p < 0.001)

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