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Fig. 2 | Virology Journal

Fig. 2

From: Molecular epidemiology of hepatitis D virus circulating in Southwestern Nigeria

Fig. 2

a Representative HDV sequences of the HBsAg-positive patients aligned with the Nigerian HDV reference sequence JX888098. HDV sequences spanning the region from nt 887 to nt 1127 showing patient-specific HDV isolates. Notably, HDV reference sequences of pSVL-D3 (Acc. No. M21012; positive control of the nRT-PCR) has been included to show that there is no cross-contamination. b Phylogenetic analysis inferred from distance analysis (Kimura 2 parameters model) using neighbor-joining bootstrap 1000 replicate reconstruction from HDV-sequences (nt 888 to nt 1122) of the Nigerian HDV isolates (highlighted in boldface and designated as NGA and numbers, e.g NGA 14-375) and the corresponding region of reference sequences showing that the Nigerian HDV isolates clustered in the HDV genotype 1 branch. The Nigerian HDV sequences were compared to HDV reference sequences gathering the 8 HDV genotypes (HDV1 to HDV8) which are denoted at the right in brackets (accession number, genotype, and geographic area are denoted in the figure). The numbers at the nodes indicate bootstrapping values. The bar represents nucleotide substitutions per position. HDV sequences of the patient isolates are available at NCBI Genebank database (Acc. No. KU844264 to KU844277)

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