Fig. 6
From: A heat-inactivated H7N3 vaccine induces cross-reactive cellular immunity in HLA-A2.1 transgenic mice
Quantification of M158-specific CD8 + T cells isolated from lung airways and lymphoid organs, and influenza-specific antibodies following PR8-NPN370Q challenge. Groups of mice were treated as described in the legend for Fig. 5. a On day 5 or day 7 post-challenge, the mice were sacrificed and the numbers of M158-specific CD8+ T cells were examined in the BAL, lungs, MLN, and spleen by using the IFN-γ-ELISPOT assay. Results are expressed as means of triplicate wells ± SD. b & c Serum samples were drawn before (pre) and 7 days post-challenge (post) from mice previously immunized with the HI-H7N3 virus, and specific antibodies against H7N3 and PR8 viruses were measured by HI (b) and ELISA (c); ELISA for IgG antibodies; values for sera were obtained with use of 100-fold-diluted samples. ND = None detected. OD 450 = Optical density at 450 nm. Values represent mean of 6 mice per group ± SD. One of three similar experiments is shown. *P < 0.05, NS = not significant, compared with pre-challenge sera