Fig. 2
From: A heat-inactivated H7N3 vaccine induces cross-reactive cellular immunity in HLA-A2.1 transgenic mice
HLA-A2-restricted influenza recall responses in mice immunized with HI-H7N3 virus and then infected with PR8 virus. Naïve mice (a) or mice vaccinated with HI-H7N3 virus 4 weeks earlier (b) (5/group) were challenged i.n. under anesthesia with 102.4 TCID50 of PR8. Seven days after challenge, mice were sacrificed and influenza-specific CD8+ T cell responses were measured in cells from freshly isolated splenocytes of individual mice by means of an ex vivo IFN-γ-ELISPOT assay with the indicated peptides. Bars represent means ± SD for five mice per group. The data are representative of two independent experiments that gave similar results