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Table 1 Primers for PTV and PICV

From: Development of real-time reverse transcriptase qPCR assays for the detection of Punta Toro virus and Pichinde virus

Virus

Primers/probe

Sequence (5’-3’)

Conc. (μM)

Amplicon

 

F3512

CATGTGTGGCCCCCATTT

0.5

63 bp

PICV

R3574

TCAGTTGTTAGGCAAAGTGGTCTT

0.5

 
 

P3532S-MGB

6FAM-AATGGTCCATTGACACGG-MGBNFQ

0.2

 
 

F430

CAGATAGCTGCTGCCATTTTACA

0.5

66 bp

PTV

R495

GCTTTTAAGTTTCCCAGCCAAA

0.5

 
 

P454S-MGB

6FAM-CTCATTATTGTGGGCTCAT-MGBNFQ

0.2

 
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Virology Journal

ISSN: 1743-422X