Fig. 1

Analytical LOD for the PTV and PICV assays. Preliminary and confirmatory LODs were performed with primer probe combinations listed in Table 1. a Preliminary LODs were determined as having 3/3 replicate Cq values below 40 using serial 10-fold dilutions of PTV and PICV RNA extracted from water. Error bars represent the standard deviation of three replicates. b Confirmatory LODs were demonstrated with 60 replicates of PTV and PICV RNA extracted from water. Numbers in parenthesis represent the number of replicates with Cq values below the cutoff line. Each replicate is shown as an individual point with the bars representing the mean and standard deviation. In each assay, replicates that had no amplification curves or Cq values falling above 40 were given a base value of 40