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Fig. 4 | Virology Journal

Fig. 4

From: Actin- and clathrin-dependent mechanisms regulate interferon gamma release after stimulation of human immune cells with respiratory syncytial virus

Fig. 4

IFN-γ release after RSV infection is dependent on T-cell receptor signaling. a IL-18 bp was added to PBMCs simultaneously with RPMI, RSV at MOI 1 or heat killed Candida. IFN-γ release was measured after 24 h. b PBMCs were pre-treated with RPMI or CsA (100 nM). After pre-treatment, PBMCs were incubated with RPMI or RSV at MOI 1 for 24 h and IFN-γ release was measured. Lower limit of detection: 20 pg/ml. Wilcoxon-signed rank test was used for statistical analysis. Data are mean ± SEM (N = 4-5). (*p < 0.05) (ND = non-detectable) (NS = no significant difference between untreated and CPZ-treated cells)

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