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Fig. 3 | Virology Journal

Fig. 3

From: Actin- and clathrin-dependent mechanisms regulate interferon gamma release after stimulation of human immune cells with respiratory syncytial virus

Fig. 3

T cell activation and upregulation of MHC-II after RSV infection are clathrin-dependent. a-d PBMCs were pre-treated with RPMI or CPZ (5 μg/ml). After pre-treatment, PBMCs were incubated with RPMI or RSV at MOI 1 for 24 h. The effect of CPZ on (a) CD4 T cell activation, (b) CD8 T cell activation and (c) MHC-I and (d) MHC-II expression on monocytes cells was determined. Stimulation with PHA (5 μg/ml) was used as positive control for T cell activation (a-b). One-way analysis of variance with Bonferroni’s Comparison Test was used for statistical analysis. Data are mean ± SEM (N = 4). (*p < 0.05,**p < 0.01,***p < 0.001). (NS = no significant difference between unstimulated and RSV-stimulated cells)

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