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Fig. 4 | Virology Journal

Fig. 4

From: Influence of the amino acid residues at 70 in M protein of porcine reproductive and respiratory syndrome virus on viral neutralization susceptibility to the serum antibody

Fig. 4

Binding analysis of the resistant and recombinant mutant strains. A binding ELISA was performed using plates coated with each of the indicated viruses in triplicate. Serial dilutions of N4 serum were added to the wells, followed by the addition of a secondary anti-pig antibody conjugated to HRP. OD values were read at 450 nm. The negative control was wells that coated with ultracentrifuged non-infected MARC-145 cell lysates. The consistent concentration of ultracentrifugal PRRSV viruses that coated on the plate were identified by using Western Blot of N proteins. a rBB and related mutant viruses. b rBB20s and related mutant viruses

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Virology Journal

ISSN: 1743-422X