Table 2 Sensitivity of primer pair TYL828F/TYL834R in the detection of Tomato yellow leaf curl virus (TYLCV) by recombinase polymerase amplification (RPA) and polymerase chain reaction (PCR) assays
Template source | Tissue source | Detection limita | |
|---|---|---|---|
PCR | RPA | ||
Cloned TYLCV genome | -- | 9.6 × 10 −15 g | 9.6 × 10−12 g |
Purified DNA | TYLCV- infected tomato | 1 × 10−9 | 1 × 10−5 |
TYLCV- infected bean | 1 × 10−6 | 1 × 10−3 | |
Non-inoculated tomato | 0 | 0 | |
Non-inoculated bean | 0 | 0 | |
Crude extraction | TYLCV- infected tomato | 0 | 1 × 10−3 |
TYLCV- infected bean | 0 | 1 × 10−2 | |
Non-inoculated tomato | 0 | 0 | |
Non-inoculated bean | 0 | 0 | |