Fig. 3From: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinicsClinical trial comparing RPA and PCR for the detection of Tomato yellow leaf curl virus (TYLCV) in field samples. Assays used primer pair TYL828F/TYL834R for the detection of TYLCV in samples collected from thirteen plants from two tomato field sites. a Amplification using RPA and b Amplification using PCR. Samples 1 through 10 had symptoms characteristic of an established infection of TYLCV while the symptoms in samples 11–13 were less characteristic. Lane M: 50 bp ladder MW standard, size is indicated in kilobases (kb); Lanes 1 – 13 samples 1 – 13; Lane 14 TYLCV-infected plant sample; Lane15 non-inoculated tomato; Lane 15 water controlBack to article page