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Fig. 3 | Virology Journal

Fig. 3

From: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics

Fig. 3

Clinical trial comparing RPA and PCR for the detection of Tomato yellow leaf curl virus (TYLCV) in field samples. Assays used primer pair TYL828F/TYL834R for the detection of TYLCV in samples collected from thirteen plants from two tomato field sites. a Amplification using RPA and b Amplification using PCR. Samples 1 through 10 had symptoms characteristic of an established infection of TYLCV while the symptoms in samples 11–13 were less characteristic. Lane M: 50 bp ladder MW standard, size is indicated in kilobases (kb); Lanes 1 – 13 samples 1 – 13; Lane 14 TYLCV-infected plant sample; Lane15 non-inoculated tomato; Lane 15 water control

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Virology Journal

ISSN: 1743-422X