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Fig. 1 | Virology Journal

Fig. 1

From: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics

Fig. 1

Detection of Tomato yellow leaf curl virus (TYLCV) by RPA and purified DNA as template. Amplification using primer pair TYL828F/TYL834R from different host plants a Lane M: 50 bp ladder MW standard, size is indicated in kilobases (kb); Lane 1 TYLCV-infected tomato; Lane 2 non-inoculated tomato; Lane 3 TYLCV -infected bean; Lane 4 non-inoculated bean; Lane 5 TYLCV-infected tobacco; Lane 6 non-inoculated tobacco; Lane 7 water control. Amplification from P. vulgaris (bean) infected with one of four different begomoviruses b Lane 1 non-inoculated, Lane 2 Bean golden yellow mosaic virus (BGYMV); Lane 3 Euphorbia mosaic virus (EuMV); Lane 4 Sida golden mottle virus (SiGMoV); Lane 5 Tomato mottle virus (ToMoV); Lane 6 TYLCV. Ten μl of amplified product (cleaned by heating to 65 °C for 10 min) were loaded into each lane of the 1.5 % agarose gels and stained with ethidium bromide

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