Fig. 1From: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinicsDetection of Tomato yellow leaf curl virus (TYLCV) by RPA and purified DNA as template. Amplification using primer pair TYL828F/TYL834R from different host plants a Lane M: 50 bp ladder MW standard, size is indicated in kilobases (kb); Lane 1 TYLCV-infected tomato; Lane 2 non-inoculated tomato; Lane 3 TYLCV -infected bean; Lane 4 non-inoculated bean; Lane 5 TYLCV-infected tobacco; Lane 6 non-inoculated tobacco; Lane 7 water control. Amplification from P. vulgaris (bean) infected with one of four different begomoviruses b Lane 1 non-inoculated, Lane 2 Bean golden yellow mosaic virus (BGYMV); Lane 3 Euphorbia mosaic virus (EuMV); Lane 4 Sida golden mottle virus (SiGMoV); Lane 5 Tomato mottle virus (ToMoV); Lane 6 TYLCV. Ten μl of amplified product (cleaned by heating to 65 °C for 10 min) were loaded into each lane of the 1.5 % agarose gels and stained with ethidium bromideBack to article page