Fig. 2From: Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virusDetermination of reaction time and temperature for the ORFV RPA-LFD assay. The assay works effectively in a broad range of constant reaction temperatures (a). After 10 min of isothermal amplification reaction, the test line is visible on the test strip. Including the incubation of 5 min the whole assay time of the ORFV RPA-LFD assay is less than 20 min (b)Back to article page