Table 1 List of primers used for identification of the genome of the Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) by RT-PCR amplification
Primer set no. | Amplicon position (nt.) | Forward primer (5′ → 3′) | Complementary primer (5′ → 3) |
|---|---|---|---|
1 | 1–1962 | TTGAAAAGATCGTGTAGGGTA | CCGGTTGTTACGGAAAGGTA |
2 | 1895–3884 | CCGTTCCATCCGATCTCTTA | CCGAATAAGTTTAACCCGTGTG |
3 | 3653–5542 | TGAAAGCTGTAATTAACTGTGTTGG | CAGGCTTACCATCAAGCCATA |
4 | 5329–7128 | AATGACATTCAGTGGCGTGA | AACGACAGGGCAGTCTTTTG |
5 | 7088–8920 | ATGCACCTTGGTGTGCCTAT | GAGCTAGTTCCGACCACTCG |
6 | 8300–10166 | TAACTCCTCGCGACCCTAGA | TTTAAAGAAAGAGCACTGTCCT |