Fig. 3

Phylogenetic analysis of Brevicoryne brassicae virus (BrBV). Rooted phylogenetic tree based on the deduced amino acid sequences of the polyproteins of some members of the genus Iflavirus. Bootstrap values of 100 replicates are indicated. For visualization we used FigTree sofrtware [17] Viral sequences (with abbreviations and NCBI/GenBank accession numbers): Heliconius erato iflavirus (H.erato iflavirus; KJ679438), Spodoptera exigua iflavirus 2 isolate Korean (SeV; JN870848), Deformed wing virus isolate Chilensis A1 (DWV Chilensis A1; JQ413340), Lymantria dispar iflavirus 1 isolate Ames (LdiV1; KJ629170), Antheraea pernyi iflavirus isolate LnApIV-02 (LnApIV-02; KF751885), Perina nuda picorna-like virus (PnPV; AF323747), Sacbrood virus strain AcSBV-Kor4 (AcSBV-Kor4; KP296803), Sacbrood virus strain AcSBV-Kor3 (AcSBV-Kor3; KP296802), Sacbrood virus strain AmSBV-Kor2 (AmSBV-Kor2; KP296801), Sacbrood virus strain AmSBV-Kor1 (AmSBV-Kor1; KP296800), Sacbrood virus isolate AmSBV-Viet6 (AmSBV-Viet6; KM884995), Sacbrood virus isolate AcSBV-Viet5 (AcSBV-Viet5; KM884994), Sacbrood virus isolate AmSBV-Viet4 (AmSBV-Viet4; KM884993), Sacbrood virus isolate AcSBV-Viet3 (AcSBV-Viet3; KM884992), Sacbrood virus isolate AcSBV-Viet2 (AcSBV-Viet2; KM884991), Sacbrood virus isolate AcSBV-Viet1 (AcSBV-Viet1; KM884990), Sacbrood virus isolate CSBV-FZ (CSBV-FZ; KM495267), Spodoptera exigua iflavirus 2 (SeV-2; KJ186788), Sacbrood virus strain SBM2 (SBM2; KC007374), Lygus lineolaris virus 1 isolate LlV-1 (LlV-1; JF720348), Sacbrood virus strain AmSBV-Kor19 (AmSBV-Kor19; JQ390592), Sacbrood virus strain AmSBV-Kor21 (AmSBV-Kor21; JQ390591), Varroa destructor virus 1 (VDV-1; AY251269), Infectious flacherie virus strain IFV-India (IFV-India; HM569717), Nilaparvata lugens honeydew virus-1 (NLHV-1;AB766259), Brevicoryne brassicae picorna-like virus isolate IL (BrBV-IL; KP777548), Sacbrood virus CSBV-LN/China/2009 (CSBV-LN/China/2009; HM237361), Dinocampus coccinellae paralysis virus strain Quebec2013 (DcPV;KF843822), Deformed wing virus isolate Varroa-infested-colony-DJE202 (DWV- DJE202; KJ437447), Sacbrood virus isolate SXnor1 (SXnor1; KJ000692), Sacbrood virus strain BJ 2012 (BJ 2012; KF960044), Deformed wing virus strain Korea-2 (DWV-Korea2; JX878305), Deformed wing virus strain Korea-1 (DWV-Korea1; JX878304), Formica exsecta virus 2 isolate Fex2 (Fex2; KF500002), Sacbrood virus strain II-9 (SBV-II-9; JX270800), Sacbrood virus strain S2 (SBV-S2; JX270799), Sacbrood virus strain K3A (SBV-K3A; JX270798), Sacbrood virus strain K5B (SBV-K5B; JX270797), Sacbrood virus strain K1A (SBV-K1A; JX270796), Sacbrood virus strain II-2 (SBV-II-2; JX270795), Deformed wing virus isolate VDV-1-DWV-No-9 (VDV-1-DWV-No-9; HM067438), Deformed wing virus isolate VDV-1-DWV-No-5 (VDV-1-DWV-No-5; HM067437), Deformed wing virus isolate PA (DWV-pa; AY292384), Infectious flacherie virus strain ZheJiang01/CHN (IFV-Z01/CHN; EU868609), Brevicoryne brassicae picorna-like virus (BrBV; EF517277), Sacbrood virus (SBV; AF092924), Kakugo virus (Kakugo virus; AB070959), Spodoptera exigua Iflavirus-1 (SelV-1; JN091707), Laodelphax striatellus picorna-like virus 2 isolate LsPV2 (LsPV2; KM272628), Graminella nigrifrons virus 1 isolate Ohio (GNV1; KP866792), Sacbrood virus strain HYnor (SBV-HYnor; KJ959614) and Sacbrood virus strain LDst (SBV-LDst; KJ959613). Acute bee paralysis virus (ABPV; NC_002548) served as outgroup