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Fig. 2 | Virology Journal

Fig. 2

From: Extended phylogenetic analysis of a new Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) suggests taxonomic revision of the genus Iflavirus

Fig. 2

Schematic representation of Brevicoryne brassicae virus (BrBV-IL) genome and illustration of the sequencing strategy. a Genomic structure of BrBV-IL genome, including the viral protein genome-linked (VPg), 5′UTR (792 nt) and 3′UTR including the 3′ polyadenylated tail (419 nt including the stop codon). The long polyprotein encodes non-structural proteins, including motifs of chymotripsin-like proteinase, NTPase/helicase and RNA-dependent RNA polymerase (RdRp), and of viral structural proteins VP1–VP4. b The strategy used for BrBV-IL genome sequencing. The rectangles below the genome map represent the locations and lengths of sequences obtained by NGS analysis of siRNA. c The sequenced clones obtained by RT-PCR amplification using six primer sets (Table 1) to map the entire viral genome

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