Human H7N9 virus induces a more pronounced pro-inflammatory cytokine but an attenuated interferon response in human bronchial epithelial cells when compared with an epidemiologically-linked chicken H7N9 virus

Table 1 Primers and conditions for real-time RT-PCR

Cytokines	Primers
	Forward	Backward	Mg conc. (mM)	Denaturation	Annealing	Extension
TNF-α	GCCAGAGGGCTGATTAGAGA	CAGCCTCTTCTCCTTCCTGAT	2	95 °C 10s	60 °C 5 s	72 °C 5 s
MCP-1	GCAATCAATGCCCCAGTCA	TGCTGCTGGTGATTCTTCTATAGCT	2	95 °C 10s	55 °C 5 s	72 °C 5 s
IL-6	GGTACATCCTCGACGGCATCT	GTGCCTCTTTGCTGCTTTCAC	2	95 °C 10s	55 °C 5 s	72 °C 5 s
IFN-β	GCCGCATTGACCATCT	CACAGTGACTGTACTCCT	4	95 °C 10s	55 °C 5 s	72 °C 11 s
IFNL1	GAAGCAGTTGCGATTTAGCC	GAAGCTCGCTAGCTCCTGTG	2	95 °C 10s	60 °C 5 s	72 °C 7 s
IL-10	CAAATGAAGGATCAGCTGGACAA	GCATCACCTCCTCCAGGTAAAAC	2	95 °C 10s	55 °C 5 s	72 °C 5 s
TGF-β	CCCAGCATCTGCAAAGCTC	GTCAATGTACAGCTGCCGCA	2	95 °C 10s	55 °C 5 s	72 °C 5 s
IL-8	AGCTGGCCGTGGCTCTCT	CTGACATCTAAGTTCTTTAGCACTCCTT	2	95 °C 10s	55 °C 5 s	72 °C 5 s
GAPDH	ATTCCACCCATGGCAAATTC	CGCTCCTGGAAGATGGTGAT	2	95 °C 10s	55 °C 5 s	72 °C 5 s