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Fig. 5 | Virology Journal

Fig. 5

From: Inhibition of protein kinase C promotes dengue virus replication

Fig. 5

Depletion of PKC increased viral-induced host cell damage. a siRNA-mediated depletion of PKCα resulted in increased intracellular DENV copy number. HepG2 cells were transfected with PKCα-specific siRNA (siPKCα) or non-targeting siRNA (siNeg) for 24 h prior to a 2-h infection of DENV strain 16681. After 24 h, cells were harvested. PKCα mRNA and DENV viral copy numbers were quantified by quantitative PCR. Left; bars represent viral copy numbers in the host cells treated with siRNA indicated. Right; bars represent means expressions of PKCα mRNA from 3 independent experiments. Error bars represent S.D., *; p-value ≤ 0.05. b Expressions of PKCα, DENV NS3, and NS5 were examined by immunoblots using indicated antibodies. GAPDH immunoblot was used as loading control. c Average DENV viral copy numbers in culture mediums from PKCα-depleted HepG2 cells (siPKCα), compared to control cells (siNeg). Bars represent means of 3 independent experiments. Error bars represent S.D. The viral copy numbers were determined by RT-qPCR. d Depletion of PKCα decreased the viability of DENV-infected cells. HepG2 cells were treated as indicated. The 24 h post transfection, cells were infected with DENV. Cell viabilities were examined using MTT assay. Graphs showed average of 3 independent experiments. Error bars represent S.D. *; p-value ≤ 0.05, **; p-value ≤ 0.01. e Inhibition of PKC by BisI decreased viability of DENV-infected cells. HepG2 cells were treated with 1 μM BisI for 30 min, prior to a 2-h infection by DENV strain 16681. Cell viabilities were determined by tryptan blue staining at indicated time points. Bars represent means of 3 independent experiments. Error bars represent S.D., *; p-value ≤ 0.05

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