Fig. 5

SULT1A1 influences the kinetics of minus-strand DNA elongation. a The step during HIV-1 reverse transcription that SULT1A1 regulates was investigated by quantitative real-time PCR analysis with the indicated primer sets. The stages of reverse transcription are shown: 1. Viral genomic RNA; 2. Minus-strand DNA initiation; 3. Minus-strand DNA transfer; 4. Minus-strand DNA elongation and plus-strand DNA initiation; 5 and 6. Plus-strand DNA transfer; 7. Plus-strand DNA elongation. b MDMs were treated with control or SULT1A1-specific siRNA and challenged with VSV-G pseudotyped NL43-Luc HIV-1 vector. Total cellular DNA was isolated at 8, 16, or 24 h post infection. Quantitative real-time PCR was performed to measure the relative abundance of DNA products corresponding to specific steps during the process of reverse transcription using primers indicated in Fig. 5a. Results shown are from MDMs derived from 6 donors assayed once. Mean and SD shown, *** p < 0.0005 ** p < 0.005, one sample t test