Genetic characterization of ØVC8 lytic phage for Vibrio cholerae O1

Solís-Sánchez, Alejandro; Hernández-Chiñas, Ulises; Navarro-Ocaña, Armando; De la Mora, Javier; Xicohtencatl-Cortes, Juan; Eslava-Campos, Carlos

doi:10.1186/s12985-016-0490-x

Virology Journal

Table 2 Putative open reading frames in the ØVC8 genome and their assigned functions

From: Genetic characterization of ØVC8 lytic phage for Vibrio cholerae O1

			Gene product
ORF	Nucleotide position		Size	^a Molecular mass	Presumed function	Related phage or organism	GenBank accession no	BLAST X
	Start	End	(aa)	(kDa)				% Identity	e-value
1	1	222	73	13.6	Hypothetical protein	Vibrio phage VP2	YP_052974	95	1 e-32
2	128	739	203	22.9	Terminase small subunit	Vibrio phage VP5	YP_053007	94	4 e-131
3	726	2432	568	65.3	Terminase large subunit	Pelagibacter phage HTVC010P	YP_007517700	32	4 e-41
4	2443	4086	547	61.7	Head-to-tail connecting protein	Pelagibacter phage HTVC010P	YP_007517703	32	4 e-62
5	4086	4313	75	8	Hypothetical protein	Vibrio phage VP2	YP_052976	92	7 e-26
6	4326	4583	85	9.9	Hypothetical protein	Vibrio phage VP2	YP_052977	95	2 e-18
7	4552	5439	295	31.8	Hypothetical protein	Pelagibacter phage HTVC010P	YP_007517705	23	6 e-14
8	5607	6575	322	36.1	Structural protein	Pelagibacter phage HTVC010P	YP_007517707	24	1 e-21
9	6642	6911	89	9.2	Hypothetical protein	Vibrio phage VP2	YP_052980	100	7 e-35
10	6926	7774	282	31.1	Hypothetical protein	Vibrio phage VP5	YP_053013	91	1 e-46
11	7771	8136	121	13.4	Hypothetical protein	Vibrio phage VP2	YP_052982	96	1 e-59
12	8138	8611	157	18.2	Hypothetical protein	Vibrio phage KVP40	NP_899538	31	2 e-20
13	8604	8906	100	10.5	Hypothetical protein	Vibrio phage VP2	YP_052983	97	1 e-51
14	8930	11155	741	74.1	Tail protein	Vibrio phage VP5	YP_024980	90	0
15	11164	12948	594	65.5	Structural protein	Vibrio phage VP5	YP_024422	98	0
16	12951	13349	132	13.9	Hypothetical protein	Vibrio phage VP2	YP_052984	97	1 e-41
17	13346	15430	694	76.8	Structural protein	Vibrio phage VP2	YP_052985	95	0
18	15430	16602	390	41.5	Hypothetical protein	Vibrio phage VP5	YP_053019	95	2 e-151
19	16604	18952	782	87.2	Structural protein	Escherichia phage phiV10	YP_512274	27	6 e-68
20	18956	19984	342	36.6	Tail fiber	Vibrio phage VP5	YP_053020	96	2 e-162
21	20009	21322	437	44.9	Outer capsid protein	Vibrio phage VP2	YP_024425	31	1 e -35
22	21350	21646	98	11.2	Hypothetical protein	Pseudomonas phage PPpW-3	YP_008873205	45	9 e-16
23	21723	22160	145	11.2	Hypothetical protein	Vibrio phage VP2	YP_053022	96	2 e-79
24	22150	22308	52	11.2	Hypothetical protein	Vibrio phage VP2	YP_052988	81	1 e-19
25	22305	22817	170	19.2	Metal dependent phosphohydrolase	Vibrio phage VP5	YP_024983	94	7 e-116
26	22819	23079	86	9.8	Hypothetical protein	Vibrio phage VP5	YP_053024	96	2 e-28
27	23081	24112	343	38.1	Adenylosuccinate synthetase	Solibacter usitatus	YP_827486	33	1 e-31
28	24154	26442	762	87	Integrase	Vibrio phage VP2	YP_024428	99	0
29	26432	28333	633	71.2	DNA Polymerase I	α-proteobacteria phage ØJL001	YP_223952	26	9 e-40
30	28330	28680	116	13.5	Hypothetical protein	Enterococcus phage EFDG1	AJP61480	53	8 e-14
31	28677	29192	171	19.2	ssDNA binding protein	Vibrio phage VP2	YP_024430	93	8 e-95
32	29253	30068	271	30.3	Hypothetical protein	Pseudomonas phage F8	YP_001294468	32	1 e-23
33	30119	30586	155	17.8	Hypothetical protein	Vibrio phage VP2	YP_052991	98	5 e-108
34	30649	32118	489	55.4	Superfamily II DNA/RNA helicases	Thermoanaerobacterium phage THSA-485A	YP_006546319	36	2 e-77
35	32188	32538	116	12.6	Hypothetical protein	Vibrio phage VP5	YP_053027	84	1 e-26
36	32538	33230	230	24.5	Hypothetical protein	Vibrio phage CJY	AIZ01434	97	3 e-111
37	33381	33845	154	17.3	Hypothetical protein	Vibrio phage VP2	YP_052993	84	6 e-93
38	33842	34252	136	15.7	Hypothetical protein	Vibrio phage VP2	YP_052994	78	2 e-64
39	34254	34613	119	13.7	Hypothetical protein	Vibrio phage VP5	YP_053031	67	4 e-44
40	34621	35421	266	30.2	Hypothetical protein	Vibrio phage VP2	YP_052996	98	2 e-101
41	35490	35975	161	18.1	Hypothetical protein	Vibrio phage VP2	YP_052977	96	5 e-85
42	35985	36437	150	16	Hypothetical protein	Vibrio phage VP5	YP_053036	84	2 e-61
43	36543	36836	97	11	Hypothetical protein	Vibrio phage VP2	YP_053001	95	1 e-46
44	36848	37138	96	10.7	Hypothetical protein	Vibrio phage VP2	YP_053002	79	5 e-36
45	37245	37490	81	9.2	Hypothetical protein	Vibrio phage VP2	YP_053003	74	1 e-26
46	37495	38094	199	22.1	Hypothetical protein	Vibrio phage VP5	YP_053042	91	5 e-93
47	38093	38788	231	26.9	Hypothetical protein	Vibrio phage VP2	YP_053005	93	1 e-50
48	38669	39028	121	13.6	Hypothetical protein	Unknown	---	---	---

^aPredicted using the Compute pI/Mw tool, Swiss Institute of Bioinformatics

Back to article page

ISSN: 1743-422X

Contact us

Submission enquiries: journalsubmissions@springernature.com