Fig. 6

Effect of NH ₄ Cl on the production of progeny virus. Cells were incubated with NH₄Cl at the indicated concentrations. a CPE of GCRV infection viewed under a visible light phase microscope. b TCID₅₀ assay of virus yield in the supernatants. Each experimental point is the sum of triplicate experiments with two titer confirmation assays. Error bars represent standard errors of the mean. Asterisks represent a significant difference from the control (unpaired t-test). c Western blot analysis. Cells were treated with NH₄Cl as described above. After 12 h of incubation, cells were lysed and processed for Western blot analysis of GCRV-VP5 protein expression with polyclonal mouse serum. GAPDH was included as an internal loading control for the Western blots. NC denotes the negative control treated with the vehicle