Fig. 5

Effect of dynasore on the production of progeny virions. Cells were incubated with dynasore at the indicated concentrations. a CPE of GCRV infection viewed under a visible light phase microscope. b TCID₅₀ assay of viral yield in harvested supernatants. Each experimental point is the sum of triplicate experiments with two titer assays. Error bars represent the standard errors of the mean. Asterisks represent a significant difference from the control (unpaired t-test). c Western blot analysis to monitor viral replication level. Cells were treated with dynasore as described above. After 12 h of incubation, cells were lysed and processed for a Western blot analysis of GCRV-VP5 protein expression with polyclonal mouse serum. GAPDH was included as an internal loading control for the Western blots. NC denotes the negative control treated with the vehicle