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Fig. 3 | Virology Journal

Fig. 3

From: Porcine parvovirus infection activates mitochondria-mediated apoptotic signaling pathway by inducing ROS accumulation

Fig. 3

PPV infection led to ROS accumulation. a Observation of ROS production after PPV infection under fluorescence microscopy. Cells were infected with PPV at 1.0 MOI and harvested at 0, 12, 24, 36 and 48 hpi. Then the cells were stained with DCFH-DA and observed under fluorescence microscope. b Quantitative analysis of ROS in PPV-infected ST cells. The relative ROS level of cells infected with PPV at 1.0 MOI at 0, 12, 24, 36 and 48 hpi were analyzed by flow cytometry. c The levels of ROS for each infectious time were calculated by the fluorescence intensity in PPV-infected cells subtracting the fluorescence intensity in mock infected cells. The data are shown as mean ± SD of three independent experiments. *, p < 0.05; **, p < 0.01 in comparison with the control

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