Phylodynamics of avian influenza clade 2.2.1 H5N1 viruses in Egypt

Table 3 Mutations in the receptor binding and antigenic sites of Egyptian H5N1 viruses from 2006-14

HA mutation	Years of detection	Virus cluster				Total
HA mutation	Years of detection	2.2.1	2.2.1.2	2.2.1.1	2.2.1.1a	Total
changes in RBS
129∆	2008–2014	-	221	-	1	222
changes in AS
P74S	2007–2009	3	-	31	38	25
S120N	2007–2014	-	88	3	4	94
S120D	2010–2014	-	128	-	-	128
I151T	2008–2014	-	223	-	4	227
D154N	2007–2014	6	154	9	37	206
R162K	2007–2014	8	169	23	17	217
R162I	2006–2013	17	1	-	-	18
R162E	2008–2011	-	1	2	18	21
R140G + S141P	2007–2011	3	-	26	29	58
R140G + S141L	2009–2010	-	-	4	4	8
S141P	2008–2014	6	9	-	3	18
129L + P74S	2008–2010	-	-	7	28	35
129∆ + S120N + I151T	2009–2014	-	86	-	-	86^a
129∆ + S120D + I151T	2010–2014	-	126	-	-	126^a

RBS the receptor binding sites, AS the antigenic sites
^aTriple mutation associated with increased binding affinity to human cell receptors
The calculation was based on a total number of 368 viruses where 2.2.1 = 75, 2.2.1.2 = 224, 2.2.1.1 = 31 and 2.2.1.1 a =38

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