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Fig. 1 | Virology Journal

Fig. 1

From: Gene expression profiling of rubella virus infected primary endothelial cells of fetal and adult origin

Fig. 1

RV replication in primary endothelial cells and RV-permissive cell lines. a RV replication kinetics in HUVEC, HSaVEC, Vero76, and A549 cells. Cells were infected with RV at an MOI of 5. At the indicated time points, cell culture supernatants or cell lysates were titered in duplicate on Vero76 cells. The data are represented as the mean of two independent experiments ± standard deviation (SD). The data were analyzed by two-way ANOVA with the Bonferroni correction for multiple comparisons (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001). b Phase contrast pictures of non-infected cells or cells infected with RV with an MOI of 10 two dpi. c Capsid protein levels of RV-infected cell types at various time points post infection. Cells were infected with an MOI of 5 and harvested at the indicated time points and analyzed by flow cytometry. Intracellular staining was carried out using a capsid-specific antibody and anti-mouse-Cy5 serum as a secondary antibody. Non-infected cells served as the control. All experiments were performed three times. Error bars represent means ± SD

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