Fig. 4
No difference in the immune cell composition in bronchoalveolar lavage in Lst1 KO and C57BL/6 N wild-type control mice. Female C57BL/6 N-Lst1 tm1(KOMP)Vlcg (n = 5-6 / time point) and wild type (C57BL/6 N) mice (n = 9-10 / time point) were infected intra-nasally with 2 × 105 FFU PR8M in 20 μl PBS. On day 3 and 5 p.i. mice were euthanized by isoflurane. Bronchoalveolar lavage (BAL) was collected via instillation of 0.8 ml PBS per mouse. BAL was immediately analyzed in the hematologic system VetScan HM5 (Abaxis). Samples of mock-infected mice were collected on day 3 post PBS treatment. Absolute numbers of white blood cells (black), lymphocytes (green), granulocytes (red) and monocytes (blue) are shown in a. Lymphocyte, granulocyte and monocyte amounts relative to total white blood cells counts are depicted in b. Mean values +/− SEM are shown. Statistical analysis was performed using non-parametric Mann–Whitney U test