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Fig. 3 | Virology Journal

Fig. 3

From: Lst1 deficiency has a minor impact on course and outcome of the host response to influenza A H1N1 infections in mice

Fig. 3

Lungs of Lst1 KO mice and C57BL/6 N wild-type mice did not show obvious histological differences after IAV challenge. Female C57BL/6 N-Lst1 tm1(KOMP)Vlcg (n = 3 / time point) and wild type (C57BL/6 N) mice (n = 3 / time point) were infected intra-nasally with 2 × 105 FFU PR8M in 20 μl PBS. Lung samples were taken on day 3 and 5 p.i.. Formalin-fixed and paraffin-embedded tissue samples were sectioned at 5 μm thickness and stained with either hematoxylin and eosin or with a monoclonal antibody against the nucleoprotein of influenza A virus (anti-NP) [26]. Similar amounts of infiltrating immune cells (a-d) and similar numbers of virus-infected cells (e-h) were observed at 3 and 5 days p. i. Immuno-histochemical staining with anti-NP antibody was detected mostly in bronchiolar regions on day 3 p.i. (i and j), whereas on day 5 p.i. virus did spread further into alveolar regions in both mouse strains (k and l). A-D: Hematoxylin and eosin staining (10× magnification); e-h: anti-NP immune-histochemical staining (20× magnification); I-L: anti-NP staining (60× magnification)

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