Fig. 3From: H2AX phosphorylation and DNA damage kinase activity are dispensable for herpes simplex virus replicationATM and ATR siRNAs moderately reduce viral DNA production. HFFs were transfected with siRNAs targeting ATM or ATR. At 72 h post-transfection, cells were infected with HSV-1 or HSV-2 (MOI 5) and harvested at 16 h p.i. a ATM and ATR transcript levels in siRNA-transfected cells were quantified using qPCR, normalized to 18S and control siRNA (Allstar, AS) by the 2–ΔΔCt method. b Protein lysates of siRNA-transfected cells were immunoblotted and probed with antibodies specific for ATM or ATR. c Viral DNA levels (vDNA) were quantified by qPCR with ICP0 primers, normalized to 18S rDNA levels and Allstar control siRNA. d HSV-1 DNA levels in HFFs transfected with pooled ATM and/or ATR siRNAs were quantified by qPCR. In panels (a), (c), and (d), grey lines indicate the average of three experiments. Asterisks indicate statistical significance relative to Allstar control at p < 0.05Back to article page