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Fig. 9 | Virology Journal

Fig. 9

From: Evidence for a biphasic mode of respiratory syncytial virus transmission in permissive HEp2 cell monolayers

Fig. 9

TUNEL assay of virus-infected HEp2 cell monolayers. HEp2 cell monolayers were either mock-infected or infected with RSV using a multiplicity of infection of 0.0002. At (a) 1 day post infection (dpi), (b) 2 dpi, (c) 3 dpi, (d) 4 dpi and (e) 5 dpi the monolayers were co-stained using anti-G and TUNEL. The stained cells were then viewed using fluorescence microscopy. The anti-G infected cell clusters (white arrows) and the increased TUNEL staining (*) are indicated (objective x20 magnification). (f) Enlarged image showing virus-induced syncytia viewed using bight field (BF), TUNEL staining, and anti-G staining at 5 dpi. The syncytia (*), TUNEL staining (**) and anti-G staining (white arrow) are highlighted. Mock infected monolayers are shown at 1, 4 and 5 dpi

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