Fig. 6

Distribution of STAT1, pSTAT1, pMAPKp38 and pJNK in the RSV-infected monolayers. HEp2 cell monolayers were mock-infected or infected with RSV using a multiplicity of infection of 0.0002. a and b At 2 days post-infection (dpi) the cells were stained using anti-N and either (a) anti-STAT1 or (b) anti-pSTAT1. The cells were viewed by IF microscopy (objective x20 magnification). The STAT1 and pSTAT1 regions in the cell monolayer are highlighted (white arrow), and the region of the monolayer containing non-infected cells is indicated (*) (c and d) HEp2 cell monolayers were mock-infected or infected with RSV using a multiplicity of infection of 0.0002. At 3 dpi the cells were stained using anti-N and either (c) anti-pMAPKp38 or (d) anti-pJNK. The cells were viewed by fluorescence microscopy (objective x20 magnification). The pMAPKp38 and pJNK regions in the cell monolayer are indicated (white arrow). The region of pMAPKp38 and pJNK staining in each image is demarcated (white outline)