Fig. 3

RSV-infection induces localized changes in the F-actin distribution in HEp2 cell monolayers. a HEp2 cell monolayers were infected with RSV using a multiplicity of infection of 0.0002 and at between 1 and 5 days post-infection (dpi) the cells were co-stained using anti-G and phalloidin-FITC (to detect F-actin). The mock-infected sample was stained at 5 dpi. The stained cell monolayers were viewed by immunofluorescence microscopy (objective x20 magnification). Individual infected cells at 1 dpi (white arrows), infected cell clusters (open white box), cell detachment (*) and syncytia (**) are indicated. b Virus-induced increased phalloidin-FITC staining at the intercellular junctions. Mock and virus-infected cells at 2 dpi were stained with anti-G and phalloidin-FITC. The increased phalloidin-FITC staining in the virus infected cells is highlighted (white arrows). The stained cell monolayers were viewed by immunofluorescence microscopy (objective 100x magnification)