Fig. 2
From: HPV 16E7 and 48E7 proteins use different mechanisms to target p130 to overcome cell cycle block
Formation of the p130-DREAM complexes in CaSki expressing p130mE7 and p130PM22/mE7. a Nuclear lysates from the untrasfected CaSki cells (CaSki) or CaSki cells transfected with pMSCV puro constructed with p130 wt, p130 mE7, p130PM22 and p130mE7/PM22 were immunoprecipitated with pre-immune serum (PI) or Lin-9 antibodies and were blotted using p130 (Santa Cruz) and HA (Roche) antibodies. The input (In) control comprised of the lysates used for immunoprecipitation. The image is representative of four independent experiments. b Flow cytometry of propidium iodide stained CaSki cells transfected with pMSCV puro constructed with p130 wt, p130mE7, p130PM22 and p130mE7/PM22 and pMSCV puro vector only as a control. The estimated percentages of cells in G1, S and G2/M phase are shown