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Table 2 Number of samples for each species that (i) tested non-negative for infectious salmon anaemia virus (ISAV) by RT-qPCR1 and that produced sequences by conventional PCR for (ii) segment 6 and (iii) segment 8

From: Discovery of variant infectious salmon anaemia virus (ISAV) of European genotype in British Columbia, Canada

Fish species

Farmed fish

Wild fish

Common name

Scientific name

n

RT-qPCR

Conventional PCR seg. 8 sequence

Conventional PCR seg. 6 sequence

n

RT-qPCR

Conventional PCR seg. 8 sequence

Conventional PCR seg. 6 sequence

Atlantic salmon

Salmo salar

334

9

18

132

    

Chinook salmon

Oncorhynchus tshawytscha

13

0

0

0

102

2

2

0

Coho salmon

Oncorhynchus kisutch

4

0

0

0

68

1

6

1

Sockeye salmon

Oncorhynchus nerka

    

256

3

7

2

Kokanee

Oncorhynchus nerka

    

1

1

1

Na

Pink salmon

Oncorhynchus gorbuscha

    

118

0

2

0

Chum salmon

Oncorhynchus keta

    

68

1

1

1

Steelhead trout

Oncorhynchus mykiss

46

2

3

0

21

0

Na

Na

Cutthroat trout

Oncorhynchus clarkii

    

18

0

83

13

Chum mackerel

Scomber japonicus

    

13

0

1

0

Pacific herring

Clupea pallasi

    

44

0

1

0

Sea louse

Lepeophtheirus salmonis

    

1

1

0

0

Total

 

397

11

21

13

709

9

29

17

  1. 1All fish tissue samples were screened by the real time RT-qPCR TaqMan® assay for ISAV. However, only some of the samples were also tested in conventional RT-PCR for segment 6 HPR or segment 8. Therefore, the numbers do not reflect a direct comparison of the 3 different RT-PCR assays. Na denotes Not applicable.
  2. 22 others with a PCR product but not sequenced.
  3. 35 others with a PCR product but not sequenced.