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Fig. 3 | Virology Journal

Fig. 3

From: Discovery of variant infectious salmon anaemia virus (ISAV) of European genotype in British Columbia, Canada

Fig. 3

Alignment of ISAV segment 8 amino acid sequences in the target region of the primer-probe set of the real time RT-qPCR TaqMan® assay for ISAV [36]. The ISAV sequences are those in Fig. 2. The amino acid sequences in the region between the two primers (Forward Primer and Reverse Primer) were aligned using CLUSTAL X with the default settings [58]. The single nucleotide mutation in the probe sequence resulted in a single amino acid change from V to D

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