TY - JOUR AU - Kibenge, Molly JT AU - Iwamoto, Tokinori AU - Wang, Yingwei AU - Morton, Alexandra AU - Routledge, Richard AU - Kibenge, Frederick SB PY - 2016 DA - 2016/01/06 TI - Discovery of variant infectious salmon anaemia virus (ISAV) of European genotype in British Columbia, Canada JO - Virology Journal SP - 3 VL - 13 IS - 1 AB - Infectious salmon anaemia (ISA) virus (ISAV) belongs to the genus Isavirus, family Orthomyxoviridae. ISAV occurs in two basic genotypes, North American and European. The European genotype is more widespread and shows greater genetic variation and greater virulence variation than the North American genotype. To date, all of the ISAV isolates from the clinical disease, ISA, have had deletions in the highly polymorphic region (HPR) on ISAV segment 6 (ISAV-HPRΔ) relative to ISAV-HPR0, named numerically from ISAV-HPR1 to over ISAV-HPR30. ISA outbreaks have only been reported in farmed Atlantic salmon, although ISAV has been detected by RT-PCR in wild fish. It is recognized that asymptomatically ISAV-infected fish exist. There is no universally accepted ISAV RT-qPCR TaqMan® assay. Most diagnostic laboratories use the primer-probe set targeting a 104 bp-fragment on ISAV segment 8. Some laboratories and researchers have found a primer-probe set targeting ISAV segment 7 to be more sensitive. Other researchers have published different ISAV segment 8 primer-probe sets that are highly sensitive. SN - 1743-422X UR - https://doi.org/10.1186/s12985-015-0459-1 DO - 10.1186/s12985-015-0459-1 ID - Kibenge2016 ER -