Fig. 3
From: Recombinant Dengue virus protein NS2B alters membrane permeability in different membrane models
Association of the NS2B protein with bacterial membranes. a Western blot analysis of NS2B protein expression in E. coli using an anti-V5 antibody. Lane 1: NS2B clone, uninduced. Lane 2: bacteria transformed with the parental vector. Lane 3: NS2B clone, induced with IPTG. Lane 4: Inclusion bodies of bacteria transformed with NS2B. Lane 5: purified NS2B protein. Lane 6: purified bacterial membranes. b and c western blot analysis of bacterial membrane fractions using a mouse polyclonal antibodies specific to-OmpCF (resident bacterial membrane protein) and α-V5. Lanes 1 and 2: purified protein NS2B. Lane 3: bacterial membrane fraction