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Table 3 Detection results for clinical specimens

From: Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay

Single infection

Co-infection

 

Number

Rate (%)

Real-time PCR/RT-PCR results

Sequencing results

Measures of agreement Kappa values

 

Number

Rate (%)

Real-time PCR/RT-PCR results

Sequencing results

Measures of agreement Kappa values

MDV

26

8.7 %

26

26

1 (p < 0.001)

MDV + ALV-A

4

1.3 %

4

4

1 (p < 0.001)

ALV-A

6

2.0 %

6

6

1 (p < 0.001)

MDV + ALV-J

25

8.3 %

25

25

1 (p < 0.001)

ALV-B

2

0.7 %

2

2

1 (p < 0.001)

ALV-B + ALV-J

1

0.4 %

1

1

1 (p < 0.001)

ALV-J

21

7.0 %

21

21

1 (p < 0.001)

MDV + ALV-J + REV

6

2 %

6

6

1 (p < 0.001)

REV

15

5 %

15

15

1 (p < 0.001)

MDV + CIAV

5

1.6 %

5

5

1 (p < 0.001)

IBDV

17

5.7 %

17

17

1 (p < 0.001)

MDV + REV

6

2 %

6

6

1 (p < 0.001)

CIAV

9

3 %

9

9

1 (p < 0.001)

ALV-J + REV

3

1.5 %

3

3

1 (p < 0.001)

ARV

23

7.7 %

23

23

1 (p < 0.001)

IBDV + ALV-J

5

1.6 %

5

5

1 (p < 0.001)

      

MDV + CIAV + ALV-J

4

1.3 %

4

4

1 (p < 0.001)

      

REV + ARV

3

1 %

3

3

1 (p < 0.001)

      

ALV-J + ARV

6

2 %

6

6

1 (p < 0.001)

      

ALV-J + IBDV

3

1 %

3

3

1 (p < 0.001)