Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay

Table 1 Primer sequences and PCR product sizes

Virus	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Amplicon size (bp)	Target region	Primer concentration (μM)
MDV	AGGTGACACTATAGAATAAGGGAGCAGACGTACTATGTAGACAA	GTACGACTCACTATAGGGATGGTAAGCAGTCCAAGGGTCA	227	meq	0.16
ALV-A	AGGTGACACTATAGAATACAAGGGGTTCCTTGGTATCT	GTACGACTCACTATAGGGATGTGCCTATCCGCTGTCA	155	gp85	0.2
ALV-B	AGGTGACACTATAGAATATCAATCACGATTCTCCCACC	GTACGACTCACTATAGGGATGTGACGCTTCGTTTACGTCTT	285	gp85	0.2
ALV-J	AGGTGACACTATAGAATACTGATGCAACAACCAGGAAA	GTACGACTCACTATAGGGAGCAGTAACATTAGTGACATACCC	204	gp85	0.2
REV	AGGTGACACTATAGAATAGACCAGGCGAGCAAAATC	GTACGACTCACTATAGGGAGGTGTAATAGGTAGGTATGGAGGA	182	gp90	0.2
IBDV	AGGTGACACTATAGAATAGGGTCAGGGCTAATTGTCTT	GTACGACTCACTATAGGGATCTGTCAGTTCACTCAGGCTTC	294	VP2	0.2
CIAV	AGGTGACACTATAGAATAAAAGGCGAACAACCGATGA	GTACGACTCACTATAGGGATGCCCTGGAGGAAAAGACC	269	VP1	0.2
ARV	AGGTGACACTATAGAATAGGACCCCTACTTCTGTTCTCA	GTACGACTCACTATAGGGAATTTCCCGTGGACGACAT	215	S1	0.16
Universal primers	AGGTGACACTATAGAATA	GTACGACTCACTATAGGGA			0.25

Universal primers sequences are underlined. Chimeric primers were synthesised using universal primers and specific primers