|
Primer
|
Sequence 5' → 3'
|
|---|
|
JEF(Forward)a
|
TTG GAATTC ATG ATA AGA GGA GGG AAT G
|
|
JER(Reverse)a
|
TTT AGATCT TTA GGC ATG CAC ATT GGT CG
|
|
JEVeFb
|
GAT CTG TCG TAGCTCTTGGGTCACAG
|
|
JEVeRb
|
GCGTTGACACCCATCCAAAGAAG
|
|
IFN-γ-Fc
|
TGGCATAGATGTGGAAGAA
|
|
IFN-γ-Rc
|
GTGTGATTCAATCAATGACGCTTA
|
|
GAPDH-Fd
|
GCCCAAGATGCCCTTCAGT
|
|
GAPDH-Rd
|
CCTTCCGTGTTCCTACCCC
|
-
aPrimers used to the clone the PrM-E gene; bPrimers used for the identification of recombinant virus PRV TK−/gE−/PrM-E+; cThe special primers for analysis of IFN-γ mRNA expression through real-time RT-PCR; d: Primers used for the control of real-time RT-PCR
