Construction and immune efficacy of recombinant pseudorabies virus expressing PrM-E proteins of Japanese encephalitis virus genotype І

Table 1 Primers used in this study

Primer	Sequence 5' → 3'
JEF(Forward)^a	TTG GAATTC ATG ATA AGA GGA GGG AAT G
JER(Reverse)^a	TTT AGATCT TTA GGC ATG CAC ATT GGT CG
JEVeF^b	GAT CTG TCG TAGCTCTTGGGTCACAG
JEVeR^b	GCGTTGACACCCATCCAAAGAAG
IFN-γ-F^c	TGGCATAGATGTGGAAGAA
IFN-γ-R^c	GTGTGATTCAATCAATGACGCTTA
GAPDH-F^d	GCCCAAGATGCCCTTCAGT
GAPDH-R^d	CCTTCCGTGTTCCTACCCC

^aPrimers used to the clone the PrM-E gene; ^bPrimers used for the identification of recombinant virus PRV TK⁻/gE⁻/PrM-E⁺; ^cThe special primers for analysis of IFN-γ mRNA expression through real-time RT-PCR; d: Primers used for the control of real-time RT-PCR

ISSN: 1743-422X