Fig. 6

Humoral immune responses against PRV in immunized mice. Serum samples were collected at weekly intervals after immunization. a Serum samples of PRV-specific antibodies using the PRV-gB Antibody Test Kit by indirect ELISA, according to the manufacturer’s instructions. b PRV Neutralizing antibodies were measured in serum samples at different times by serum neutralization test. PRV Neutralizing antibodies were calculated and expressed as the reciprocal of the highest serum dilution that inhibits 50 % of the culture wells of the PRV replication. Data represent the mean ± SEM. Statistical significance was assessed by Student’s t-test (***: P < 0.001). Data represent the mean ± SEM. Different letters (a, b, c) indicate a statistically significant difference between the different experimental groups (P < 0.05)