Fig. 3
The biological characteristics analysis of PRV TK−/gE−/PrM-E+. a PRV TK−/gE−/PrM-E+ was grown on PK-15 cells sequentially for 20 passages and the total DNAs from virus-infected PK-15 cells were amplified by PCR with a specific primers for JEV E gene according to JEV SX09S-01 strain and analyzed by electrophoresis. M: marker DL2000; 1 ~ 11: cells infected with passages 11 to 20; 12: cells infected with PRV TK−/gE−/LacZ+; 13: H2O; b Genetic stability of protein identification for different generations by western blotting. PK-15 cells infected with different generations of PRV TK−/gE−/PrM-E+ were collected for JEV E protein expression detection by western blotting. 1 ~ 7: cells infected with passages 1, 2, 4, 8, 12, 16 and 20 of PRV TK−/gE−/PrM-E+. c Virus growth curves based on viral yield of supernatants harvested at different time points of PRV TK−/gE−/PrM-E+ or PRV TK−/gE−/LacZ+ infected PK-15 cells, and viral yield was measured by TCID50