Fig. 2
Identification of PRV TK−/gE−/PrM-E+ and expression of JEV PrM-E in PK-15 cells. a The total DNAs from virus-infected PK-15 cells were amplified by PCR with a pair of specific primers for JEV E gene according to JEV SX09S-01 strain and analyzed by electrophoresis. M: marker DL2000; 1 ~ 13: cells infected with different recombinant PRV clones; 14: cells infected with PRV TK−/gE−/LacZ+; 15: H2O; 16: positive control, plasmid pIE-CAG-PrM-E-BGH. b & c PK-15 cells infected with PRV TK−/gE−/PrM-E+ or parental virus PRV TK−/gE−/LacZ+ strain were collected for JEV E (B) and PrM (C) protein expression detection by western blotting. The proteins were detected in cells at 72 h post infection with the recombinant virus. Primary antibody is mAbs for E or for PrM and secondary antibody is goat anti-mouse IgG-HRP. Lane 1: cell lysates of PRV TK−/gE−/PrM-E+; Lane 3: cell lysates of PRV TK−/gE−/LacZ+