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Table 3 Primers used to confirm in vivo expression of 25 proteins identified by iTRAQ analysis of cucumber plants infected with CGMMV

From: Quantitative proteomics identifies 38 proteins that are differentially expressed in cucumber in response to cucumber green mottle mosaic virus infection

Protein name

Primer sequence (5’-3’)

Annealing temperature (°C)

Q4VZK4

TGAGACGACCCAGAAAGCACT

59.6

 

GCACAAATCAAACGAACCAAC

58.1

P62776

AAGGAGGAGCAAAGAGACACA

56.5

 

GGCATAAACAACATCCATAGCAGT

60.4

P08216

ATGGAGATGGGCTTGGTGTGA

62.3

 

GCTGTGCATTGCCTTGTGAAC

61.4

Q4VZI6

AGAAGATTCAAGAGGCCCCTA

57.5

 

GGCTCAAACAGAAACACCCAA

60.0

Q4VZH2

CTTCCATTCCATTCCTTTCCA

59.1

 

CATTACTCTCCTTTTTCTTGCG

56.8

C1M2W0

CAAAAGTGGGCTAAGAGGAGG

58.5

 

TGTCGATGATGTAGTCAAGAGAGT

55.9

Q2QD76

ACCTTTCCTATTCTTTCCCCG

59.4

 

TACTTACTATCCCTGCTGCTGT

54.2

Q84V66

AGGTACACGGGAAAAGAAGAG

55.6

 

TCGGACAGAACAGAGATCAAA

55.4

A3F9M6

ACCCTTTCACTCCCTTTTCAG

57.7

 

CATCCTTCCCACCTTGTTTCT

58.2

A4GWU2

ACTGGTGGTTTTGAGGCTGGT

58.0

 

CTTGGAGTATTTGGGTGTGGT

56.0

Q9SXL9

GGGATGTTCAGAAGAAATGGG

58.5

 

GAAAGTCGAAAGAGCAAAAGGA

58.5

A1BQK8

GGATGAAGGTGTAGTTGATGAA

54.3

 

ATGGAAAAGGGCAGAGGATAG

57.8

Q9FRW1

AAGTGCCTGTCCTTCTTGTGC

59.3

 

TGTTACTCTCGGGAATGGGTT

58.7

B1PE19

TCTTGGAAGTAGTGAGGGTGTT

55.7

 

ATCTGGCAGTCAGTAATGGTG

54.9

Q8RW69

ATGACTTCGGTGTTGGTTTGT

56.9

 

CTATGCTGTTGGTGCTTGTTT

55.7

P19135

TTTGGTACTGTGTTTGATGATGGC

61.7

 

TCGTGAAAATGGAGACGGATG

60.7

P83966

CTTGAGATGAACAAAGGGGGG

61.0

 

CACGATAGATCGAAAACGCGC

63.3

B6V8E7

CAGTTCATTCCACACCCTATC

54.3

 

GTGCTCATTTTCTTGACCCTT

56.2

P29602

AGACTCGACGAACTCGGCATG

62.5

 

CGGAGAAAGTGAGATAAAGGG

56.1

Q2MK92

GGCGATAAATTAGGAAACAGC

56.6

 

AATCACAACCAAGAAGAAGGG

56.0

Q8LK13

TCCTACTTCCAAAATCCCTCA

56.6

 

TATTGCTTCAACTTTCCACTTATAT

55.3

O04379

TATTTTACAGGGATGGGGTTAG

56.0

 

ACTGGAGGTTGGTAGTTTGGT

55.4

Q94G09

GTCAGTCATCTTCTGTCTTCGTT

55.4

 

TCATTACTCGTGCTGCGTGTT

59.2

A1BQL5

TCGTCAACATAGCATTTCTCTCATC

60.6

 

AACGCCATCCAAAACCGCAGC

68.9

Q5DJS5

CATTCTGCCTTTGTGCTTTTTC

59.6

 

ATTGATCGTCACGGTCTCGCC

64.4

Tubulin

CAAGGAAGATGCTGCCAATAA

58.0

 

CCAAAAGGAGGGAGCCGAGAC

60.0