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Table 2 Evaluation of the specificity of ORFV exo PRA assay

From: Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus

Virus family

Virus specie

Virus strain

exo RPA

qPCR

Poxviridae

ORFV

ORFV/Vaccine/CHA

5 min

12(CT)

ORFV

ORFV/Gansu/CHA

3 min

9(CT)

ORFV

ORFV/HB/CHA

4 min

11(CT)

Poxviridae other than ORFV

Capripox

Capripox virus/China Vaccine

neg

neg

Capripox

Capripox virus/Henan/CHA

neg

neg

Paramyxovirinae

PPRV

Nigeria 75/1

neg

neg

Picornaviridae

FMDV

FMDV/O/CHA

neg

neg

FMDV

FMDV/A/CHA

neg

neg

FMDV

FMDV/Asia1/CHA

neg

neg

  1. neg: negative