Fig. 1

RNAi induced by dsPns4 inhibited RDV infection in cultured leafhopper cells in vitro. a siRNAs were active in the cultured leafhopper cells transfected with synthesized dsGFP or dsPns4 at 72 h post-transfection. 5.8S rRNA was used as a control to indicate loading of equal amounts of RNA in each lane. b The infection of RDV was blocked by RNAi induced by dsPns4 in cultured leafhopper cells. At 36 hpi, cells were immunolabeled with Pns4-FITC (green) and Pns12-rhodamine (red). The enlarged images displayed green fluorescence (Pns4-FITC) and red fluorescence (Pns12-rodanmine) of the merged images in the boxed areas in each panel, indicating that Pns4 distributed at the periphery of viroplasms. Bars, 20 μm. c The treatment of dsPns4 significantly reduced the synthesis viral proteins in cultured leafhopper cells with Western blotting assay at 36 hpi. Proteins separation by SDS-PAGE was performed to detect Pns4, Pns12 or P8 with Pns4-, Pns12- or P8-specific IgGs, respectively. Actin was used as a control and was detected with actin-specific IgG