Fig. 5
From: Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana
Analysis of VP6/8*(N/C) expression over time using two different expression vectors, pRIC 3.0 and pTRAc-HT. a VP6/8*C and b VP6/8*N expression analysis. Proteins were detected by immunoblotting using anti-VP6 (1/5000) R – pRIC 3.0 + VP6/8*. T – pTRAc-HT + VP6/8*. M – Pre-stained molecular marker, +ve – pTRAc-VP6. –ve – plants with infiltration media only. Arrows indicate expected band size (VP6/8*(N/C) ± 43 kDa, VP6 ± 42 kDa)