Fig. 4
From: Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana
Expression of the chimeric protein VP8*/6. a Western blot analysis of pEAQ-VP8*/6 expression in N. benthamiana over time at OD600.0,25 and 0,5. b Western blot of plants infiltrated with either VP8*/6 or VP6 reveals a common band of roughly 45 kDa. Proteins were detected using commercial anti-VP6 antibody (1/5000). M – Pre-stained molecular marker, −ve – plants with infiltration media only. Arrows indicate expected band size (VP8*/6 ± 73 kDa, VP6 ± 42 kDa)