Fig. 2
From: Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana
VP6 accumulation in the cytoplasm, ER, chloroplast and apoplast. Different vectors were used to target VP6 to intracellular compartments: a pTRAc-VP6 (cytoplasm), b pTRAkc-ERH-VP6 (Endoplasmic Reticulum), c pTRAkc-rbcs-cTP-VP6 (chloroplast targeting) and d pTRAkc-A-VP6 (apoplast). Western blot was performed to access expression levels of rotavirus VP6 protein in plant leaf cell compartments over 7 days. Mouse anti-rotavirus VP6 antibody (1:5000) was used to probe the membranes. (+) and (−) indicates expression with or without silencing suppressor NSs, respectively. Arrows indicate the position of VP6 proteins in the various samples analysed (±42 kDa). M – Pre-stained molecular marker, −ve – plants infiltrated with NSs only