Table 1 Primers used in this study
Primer | Sequence of oligonucleotide (5′ → 3′) | Application |
|---|---|---|
TLR3-F | AGCCATGGGAAGTGATATTCT | Gene cloning |
TLR3-R | CGCTCACCGTGCTTTACTATTAGAT | |
3′ RACE outer primer | TACCGTCGTTCCACTAGTGATTT | RACE PCR |
TLR3-3GSP1 | GGATAGCTTTCTACTGGAACATT | |
3′ RACE inner primer | CGCGGATCCTCCACTAGTGATTTCACTATAGG | |
TLR3-3GSP2 | AACGGGACTTTGAAGCAGGCGTATC | |
5′ RACE outer primer | CATGGCTACATGCTGACAGCCTA | |
TLR3-5GSP1 | GTTGCAATCCTAAATTTGCTGACTT | |
5′ RACE inner primer | CGCGGATCCACAGCCTACTGATGATCAGTCGATG | |
TLR3-5GSP2 | CAGGGGCAAATTTTTGCACAGTTC | |
TLR3-5GSP1 | GTTGCAATCCTAAATTTGCTGACTT | |
qTLR3-F | ATGTCATGCAAACCTGACCA | Real-time quantitative PCR |
qTLR3-R | CCAGGGTCTTGAAAGGATCA | |
qNFκB-F | TGTCAGCCTTCTGGATCGCCACG | |
qMx-F | CATGGTTGTGAAGTGTCGTGGTC | |
qMx-R | TATTGTGGGTGTGCCTCGTCTGT | |
qNFκB-F | TGTCAGCCTTCTGGATCGCCACG | |
qNFκB-R | GCCCGCCAAGGGGATGTTTTCTA | |
qIFN-α-F | GGGCCCCGCAACCTT | |
qIFN-α-R | CTGTAGGTGTGGTTCTGGAGGAA | |
qISG12-F | AAAATGGCTGACCGAAACGT | |
qISG12-R | TGTGTGAAGCAAGCGAACCT | |
qOASL-F | GACCAACACACACTGCAGCACTA | |
qOASL-R | CCGTAGCCGCAGAAGCA | |
qIFITM1-F | CACCGCCAAGTACCTGAACA | |
qIFITM1-R | CGATCAGGGCGATGATGAG | |
qACTß-F | CCCCATTGAACACGGTATTGTC | |
qACTß-R | GGCTACATACATGGCTGGGG |