Fig. 1

HSV-1 UL41 protein abrogated the antiviral activity of hZAP. a and b 293Trex-hZAPL cells and 293Trex-hZAPS cells were infected with HSV-1-BAC-Luc at an MOI of 1. Cells were treated or mock treated with tetracycline (100 ng/mL or 1000 ng/mL) 2 h post-infection to induce hZAP expression. The cells were lysed, and luciferase activities were measured at 36 h after infection. Fold inhibition was calculated as the ratio of the luciferase activity in mock-treated cells to that in tetracycline-treated cells. c 293Trex-hZAPS cells were transfected with 500 ng of NL4-3-Luc reporter plasmid, together with Renilla luciferase plasmid pRL-TK (50 ng) and pEYFP-N1 control vector or plasmids encoding the indicated viral proteins (1000 ng). At 6 h post-transfection, cells were treated with or without tetracycline (1000 ng/mL) to induce hZAPS expression and were incubated for additional 36 h, followed by cell lysis. The luciferase activity was determined by a dual-luciferase assay. Data shown are means ± SD from three separate experiments. (*P < 0.05)